FAQs
Top Questions About Pipettes
Which pipette should I buy for my lab work?
Choose a pipette based on your application:
- Molecular biology → Use adjustable single-channel pipettes (0.5–10 µL, 2–20 µL, 20–200 µL ranges).
- Cell culture → Use adjustable pipettes and multichannel pipettes for 96-well plates.
- Protein work → Choose pipettes that handle a wide range (e.g., 2–20 µL, 20–200 µL, 100–1000 µL).
If you have many repetitive tasks, consider electronic pipettes - both adjustable volume and repeater pipettes.
What’s the difference between single-channel and multichannel pipettes?
- Single-channel pipettes: Move liquid one sample at a time — ideal for small-scale or varied tasks (like PCR setup, manual sample prep).
- Multichannel pipettes: Move 8 or 12 (or even more) samples at once — perfect for 96-well plates, ELISA, high-throughput experiments.
If you’re working with plates or repetitive layouts, multichannel saves huge time and reduces repetitive strain.
What’s the difference between manual and electronic pipettes?
Should I choose an air displacement or positive displacement pipette?
It depends on application and liquids you are working with.
Air Displacement Pipettes:
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Use an air cushion to aspirate and dispense liquids.
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Ideal for standard aqueous solutions.
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Higher risk of contamination, especially with sticky or evaporative liquids.
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Commonly used for general lab work, PCR, ELISA, and routine pipetting.
Positive Displacement Pipettes:
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Operate with direct liquid contact; no air cushion involved.
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Perfect for viscous, volatile, or high-density liquids.
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Lower risk of contamination, as liquid is contained in a disposable capillary.
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Frequently used for oil sampling, glycerol, radioactive materials, and biohazard samples.
Are adjustable-volume pipettes better than fixed-volume ones?
Adjustable-volume pipettes offer more versatility for various applications, while fixed-volume pipettes are optimized for a single volume, reducing potential error and improving precision.
Adjustable-Volume Pipettes:
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Allow users to set different volumes for flexible use.
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Ideal for labs with varying experimental requirements.
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Suitable for multi-purpose applications like PCR, ELISA, and serial dilutions.
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Require careful adjustment to ensure accuracy at each volume change.
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Common in research labs where flexibility is crucial
Fixed-Volume Pipettes:
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Designed to dispense a single, predefined volume accurately.
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Ideal for repetitive tasks where the same volume is consistently required.
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Minimizes user error since there is no need for volume adjustment.
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Requires multiple pipettes if different volumes are needed.
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Typically used in clinical labs, quality control, and standardized testing.
Is it safe to buy refurbished pipettes?
Yes, if you buy from a reputable source.
Good refurbished pipettes should:
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Be fully disassembled, cleaned, and rebuilt.
At Pipette.com each pipette undergoes a thorough refurbishment process conducted by certified technicians. This includes disassembly, inspection, replacement of worn components, and recalibration to ensure the pipette meets manufacturer specifications. The goal is to restore the pipette to a condition that is as close to new as possible.
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Come with calibration certificates.
At Pipette.com each refurbished pipette comes with a Certificate of Conformity, certifying that it meets technical, accuracy, and precision requirements at the time of certification. Also Pipette.com offers ISO 17025-accredited calibration services, ensuring that pipettes meet international standards for accuracy and precision. -
Offer a warranty.
At Pipette.com refurbished pipettes come with a 1-year warranty, providing customers with confidence in their purchase. Pipette.com's customer support team is available to assist with any issues or concerns that may arise.
What are the top 5 brands for pipettes?
Leading brands include Eppendorf, Gilson, Rainin, Sartorius, and Thermo Fisher. Each offers unique strengths in terms of accuracy, ergonomics, and durability.
How important is ergonomic design in pipette selection?
Ergonomics is crucial for reducing repetitive strain injuries, especially in high-throughput settings. Micropipetting for long periods can lead to hand fatigue or even a repetitive strain injury, which can affect your precision. Modern designs focus on lighter materials, comfortable grips, and smoother plunger action to minimize hand fatigue.
How Do Pipettes Work?
Pipettes are laboratory tools used to measure and transfer precise volumes of liquid. They work based on the principle of air displacement or positive displacement, depending on the type of pipette.
1. Air Displacement Pipettes (Most Common Type):
Air displacement pipettes, like single-channel, multi-channel, and electronic pipettes, operate with an internal air cushion that separates the liquid from the piston.
How It Works:
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Plunger Pressed Down:
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Pressing the plunger displaces the air inside the pipette, creating a vacuum.
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Tip Immersed in Liquid:
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When the plunger is released, the vacuum draws liquid into the tip, matching the volume set on the pipette.
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Dispensing the Liquid:
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Pressing the plunger again expels the liquid from the tip.
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Blowout Step:
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A final push expels any remaining droplets to ensure accurate transfer.
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Applications: common for aqueous solutions, biological samples, and routine lab work.
2. Positive Displacement Pipettes:
These pipettes have a direct-contact piston with the liquid, eliminating the air cushion. The piston moves inside the disposable capillary tip, displacing the liquid directly.
How It Works:
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Direct Contact:
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The piston touches the liquid directly, allowing precise control without air gaps.
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No Air Cushion:
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There’s no risk of compression errors, making it highly accurate for viscous, volatile, or high-density liquids.
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Dispensing:
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The piston pushes the liquid out completely without residue.
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Applications: ideal for viscous liquids (e.g., glycerol, oils) and volatile solvents that would evaporate with air displacement.
How to Use a Micropipette?
A micropipette works fairly simply: a vacuum is created inside the pipette to allow for precise aspiration and dispensing of small volumes of liquid.
Here are the five steps to follow when using a micropipette:
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For Adjustable Pipettes, Set the Volume
- The dial or knob on the pipette allows for volume adjustment. The user sets their desired volume for the liquid they want to aspirate, measured in microliters (µL). Fixed-volume pipettes do not contain this feature, thus the step may be skipped.
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Attach a Micropipette Tip
- Disposable pipette tips attach to the end of the pipette to aspirate and dispense liquid. Different volumes of liquid require different tips to ensure accuracy and avoid contamination.
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Aspirate the Liquid
- To aspirate, or “suck up”, the liquid one starts by depressing the plunger to the first stop (a preset resistance point) and then inserting the pipette tip into the liquid.
- Next, releasing the plunger creates the necessary vacuum to draw the liquid up into the pipette tip. The volume of liquid that is drawn will match the volume setting on the micropipette.
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Dispense the Liquid
- To release the liquid from the micropipette, the user places the tip into whichever vessel they want to dispense the liquid and the plunger is pressed past the first stop to the second stop.
- The liquid expels from the tip, with the second stop ensuring all liquid is fully ejected.
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Eject the Tip
- After dispensing the liquid, the user presses the tip ejector button to discard the used tip. This avoids contamination of the pipette itself.
We’re here to help. Below are the top 5 most common questions we hear from labs like yours—and how we can help.
Top Questions About Lab Consumables and Accessories
Can I use any tips with my pipette?
Not always.
While many pipettes are "universal," tip fit matters for accuracy and ergonomics.
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Use the same brand tips when possible for best fit (Eppendorf pipettes with Eppendorf tips, etc.).
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If using third-party tips, make sure they're low-retention and tight-fitting but don't require too much force to load or eject.
Some manufacturers offer pipettes with systems that allow height adjustment of the ejector shaft to accommodate various tip brands for optimal sealing.
What are the differences between filtered and non-filtered pipette tips?
Are there universal pipette tips for all pipette brands?
Not always. While many tips are marketed as universal, compatibility can vary. It's crucial to check the manufacturer specifications to ensure proper fitting and optimal performance.
How should I store pipette tips?
Keep tips in a clean, sealed container to maintain sterility and prevent dust contamination.
What are LTS Tips?
LTS (LiteTouch System) tips are pipette tips specifically designed for use with Rainin LTS pipettes. These are part of a system developed by Rainin (a Mettler Toledo brand) to:
- Allow minimal friction during loading and ejecting.
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Minimize strain and the risk of repetitive stress injuries.
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Ensure a perfect seal with minimal effort.
LTS pipettes have an ejector collar that ensures smooth tip ejection without excessive force.
What Is The Difference Between Rack, Reload, And Bulk Packaging For Pipette Tips?
The difference between Rack, Reload, and Bulk packaging for pipette tips primarily lies in how the tips are organized and accessed during pipetting, as well as considerations for convenience, waste reduction, and sterility.
Rack packaging provides pipette tips pre-arranged in a sturdy, plastic rack with a protective lid. It offers maximum convenience and sterility, making it ideal for applications where contamination control is crucial. The organized layout allows for quick access and compatibility with multichannel pipettes, although it generates more plastic waste and is slightly more expensive.
Reload packaging includes pipette tips in refillable trays designed to fit into reusable racks. This format significantly reduces plastic waste while maintaining organization and sterility. It is a cost-effective and eco-friendly option for labs that want to balance convenience with sustainability.
Bulk packaging supplies pipette tips loosely in a large bag or box, offering the most cost-effective and minimal waste solution. However, it requires manual handling, which increases the risk of contamination and is less suitable for sterile or high-precision applications. This option is ideal for high-throughput environments where cost savings are prioritized.
What Do I Use Extra Long Tips For?
Extra long pipette tips are designed for applications that require extended reach and precision. They are ideal for accessing samples in deep vessels, such as tall tubes, narrow flasks, and deep-well plates, without tilting or contaminating the pipette shaft. These tips also minimize cross-contamination by keeping the pipette barrel farther from the sample. Additionally, they are perfect for layering liquids in density gradients or buffer layering, as well as achieving high accuracy in small-volume pipetting.
Their unique design makes them an essential tool for precise, contamination-free pipetting in hard-to-reach areas.
What Are Low Retention Tips?
Why Do I Need A Pipette Stand?
A pipette stand is an essential accessory for organizing, protecting, and extending the life of your pipettes. It serves multiple important purposes in the lab environment:
1. Organization and Accessibility:
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Keeps your pipettes neatly arranged and easy to access.
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Prevents clutter on lab benches, especially when using multiple pipettes.
2. Prevents Contamination:
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Properly stored pipettes are less likely to come into contact with contaminated surfaces.
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Minimizes the risk of cross-contamination between samples.
3. Prevents Damage:
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Laying pipettes on their side can cause liquid residue to enter the internal mechanisms, leading to damage and inaccurate measurements.
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A stand holds them vertically, ensuring no liquid seeps back into the body of the pipette.
4. Extends Lifespan:
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Storing pipettes correctly reduces wear and tear, protecting internal components.
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Prevents mechanical issues that can arise from improper handling.
5. Efficient Lab Workflow:
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Easy to grab and replace pipettes during quick operations.
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Keeps your workspace tidy and organized for better efficiency.
We’re here to help. Below are the top 5 most common questions we hear from labs like yours—and how we can help.
Top Questions About Calibration and Maintenance
How often should I calibrate my pipette?
What are the signs that a pipette needs calibration?
Can I calibrate my pipette myself?
How quickly can you complete my pipette calibration?
We offer fast turnaround—typically 3–5 business days, depending on batch size—with free shipping both ways. This minimizes downtime and keeps your lab running smoothly.
Can I ship pipettes in batches to maintain productivity in my lab?
Absolutely! Sending pipettes in smaller batches helps keep your lab productive. Just include a copy of your quote and order form with each batch. Once your current batch is returned, you're welcome to send the next. It’s simple, efficient, and cost-effective.
What’s the right calibration service level for my pipettes?
Our specialists will help you choose the best fit:
- Calibration
- Calibration Plus
- Accredited
- Verification (ideal for quick performance checks between full calibrations)
Each option is designed to support your lab’s specific quality and compliance needs.
Is it possible to calibrate pipettes onsite at my facility?
Yes! Pipette.com offers flexible onsite calibration for labs with 50–100 pipettes or more.
Our certified technicians come directly to your facility—eliminating shipping logistics and reducing downtime. Request quotes for both onsite and in-lab services to see what works best for you.
What if my pipettes need repairs or replacement parts?
No problem. If we identify repairs during calibration, we’ll notify you before proceeding. Just check the "Notify me if repairs exceed" box on the order form—we’ll reach out so you can decide how to move forward.
Why is my pipette not dispensing accurate volumes?
We’re here to help. Below are the top 5 most common questions we hear from labs like yours—and how we can help.
Top Questions About Lab Products
What is the difference between a centrifuge and a microcentrifuge?
The main difference between a centrifuge and a microcentrifuge lies in their size, capacity, and specific applications.
A centrifuge is generally larger than a microcentrifuge and can handle sample volumes ranging from a few milliliters to several liters. Centrifuges are commonly used in clinical labs for blood separation, large-scale purification of cells, and industrial applications. They come with various rotor types like swing-bucket and fixed-angle rotors and typically operate at speeds between 1,000 and 15,000 RPM.
A microcentrifuge is a smaller version specifically designed for spinning small liquid samples, usually between 0.2 mL and 2.0 mL per tube. It is mainly used in molecular biology, biochemistry, and small-scale research for tasks such as DNA and RNA extraction, protein isolation, and quick spin-downs of small liquid volumes. Microcentrifuges generally use fixed-angle rotors and operate at higher speeds, ranging from 10,000 to 20,000 RPM.
In summary, use a centrifuge for larger samples and clinical applications, while a microcentrifuge is ideal for small-scale DNA, RNA, and protein work.
How do I balance a centrifuge properly?
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Even Number of Tubes: Always use an even number of tubes, placed directly opposite each other in the rotor.
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Equal Volumes: Ensure that the tubes opposite each other have the exact same volume and weight. Differences can lead to imbalance and potential damage.
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Symmetrical Positioning: If you have more than two tubes, distribute them symmetrically around the rotor (e.g., 3 tubes at 120° apart, 4 tubes at 90° apart).
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Balance with Water if Needed: If you have an odd number of samples, use a "balance tube" filled with water or a similar liquid to match the weight.
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Check the Rotor Lock: Before starting, ensure that the rotor is locked in place securely.
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Gradual Speed Increase: Start at a lower speed and gradually increase if needed, observing for any vibrations.
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Stop if Unstable: If you hear noise or feel vibrations, stop the centrifuge immediately and rebalance.
Proper balancing prevents equipment damage, reduces noise, and ensures accurate separation of samples.
What is the difference between refrigerated and non-refrigerated centrifuges?
The main difference between refrigerated and non-refrigerated centrifuges lies in their ability to control temperature during centrifugation:
Refrigerated Centrifuges
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Equipped with cooling mechanisms to maintain low temperatures (typically between -20°C and 40°C).
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Ideal for temperature-sensitive samples like proteins, nucleic acids, and live cells.
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Prevents sample degradation caused by heat generated during high-speed spinning.
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Commonly used in molecular biology, biochemistry, and clinical labs.
Non-Refrigerated Centrifuges
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Operate at room temperature, without cooling capabilities.
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Suitable for samples that are not sensitive to heat, such as basic separations or non-biological samples.
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More compact and typically less expensive than refrigerated models.
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Commonly used for routine sample separation in chemistry and basic biology labs.
Choose a refrigerated centrifuge for sensitive biological samples that require cooling and a non-refrigerated centrifuge for standard lab work where temperature control is not critical.
How do I choose the right rotor for my centrifuge?
To choose the right rotor for your centrifuge:
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Use a fixed-angle rotor for pelleting (cells, organelles).
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Use a swing-bucket rotor for gradient separations (blood, viruses).
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Match the rotor to your tube size, speed (RPM), and temperature needs.
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Ensure material compatibility with your samples and chemicals.
How do I properly clean and maintain laboratory glassware?
To properly clean and maintain laboratory glassware, follow these steps:
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Initial Rinse: Rinse glassware immediately after use with warm water to prevent residue buildup.
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Washing: Use a non-abrasive lab detergent and a soft brush to clean thoroughly.
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Thorough Rinsing: Rinse with tap water, then follow up with deionized or distilled water.
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Specialized Cleaning: Use acid, alkaline, or organic solvent rinses for stubborn residues, as needed.
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Drying: Air-dry inverted on a rack or use a low-temperature oven.
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Storage: Store in a clean, dust-free environment, avoiding stacking fragile items.
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Regular Maintenance: Inspect for cracks and calibration accuracy regularly.
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Safety: Always wear PPE and handle chemicals safely, preferably in a fume hood.
This routine ensures your glassware is clean, safe, and ready for accurate lab work.
How do I choose the right lab equipment for my experiment?
Choosing the right lab equipment starts with understanding your experiment's specific requirements, including the type of analysis, sample size, and the tasks you need to perform (e.g., measuring, heating, mixing). Make sure the equipment you select is compatible with the materials you're working with and suitable for the intended method. Prioritizing accuracy, safety, and efficiency will help ensure successful results.
Pipette.com Team can help you choose the best equipment with all the requirements, just contact us!
What are the essential pieces of equipment for a basic biology/chemistry lab?
Essential Equipment for a Basic Biology/Chemistry Lab:
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Glassware: Beakers, flasks (Erlenmeyer and volumetric), test tubes, and graduated cylinders for measuring and mixing liquids.
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Measuring Equipment: Pipettes (manual or electronic), burettes, and analytical balances for precise measurements.
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Heating Equipment: Bunsen burners, hot plates, and water baths for heating samples.
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Mixing Equipment: Magnetic stirrers, vortex mixers, and shaker tables for thorough mixing.
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Safety Gear: Lab coats, goggles, gloves, and fire extinguishers for protection.
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Storage and Cleaning: Test tube racks, drying racks, and chemical storage cabinets.
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Microscopy (Biology Labs): Compound microscopes for observing cell structures and small organisms.
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Centrifuge (Biology Labs): For separating cellular components or proteins.
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pH Meter (Chemistry Labs): For measuring the acidity or alkalinity of solutions.
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Fume Hood (Chemistry Labs): For safely working with volatile or hazardous chemicals.
These items form the foundation of most experiments and ensure accurate, safe, and efficient laboratory work. It is also important to consider "green lab" practices when selecting your equipment.
What is preventive maintenance for lab equipment?
Preventive Maintenance for Lab Equipment
Preventive maintenance is the routine care and servicing of laboratory equipment to prevent unexpected failures and extend its lifespan. This includes:
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Regular Cleaning: Removing dust, spills, and residues to prevent contamination.
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Calibration: Ensuring accurate measurements for equipment like pipettes and balances.
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Inspection: Checking for wear, damage, and proper functioning of parts.
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Lubrication: Applying appropriate lubricants to moving parts where necessary.
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Software Updates: Keeping any software-controlled equipment up-to-date.
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Replacement of Consumables: Changing filters, seals, and gaskets regularly.
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Documentation: Logging maintenance activities and any issues found.
Preventive maintenance helps maintain accuracy, safety, and efficiency in the lab, minimizing downtime and costly repairs.
We’re here to help. Below are the top 5 most common questions we hear from labs like yours—and how we can help.
Shipping & Return
How To Return?
To begin the Return Authorization / Exchange process please complete our “Return / Exchange Request”. Your return authorization request will be processed within 5 business days.
Return & Exchange Policy:
- You have 10 business days after delivery to initiate the return / exchange by completing our “Return / Exchange Request”.
- After the return / exchange is approved, you will be sent an RMA form that must be included with your return / exchange. The product should be shipped back within 10 business days.
- Returned / exchanged items must be unopened, and in the original packaging. Manufactured sealed Tips must be returned by pack or case. No opened packs will be accepted.
- Drop shipped items. Products shipped to you directly from the manufacturer is only eligible if the Manufacturer accepts the return / exchange. Please contact us by submitting our “Return / Exchange Request”.
- Products shipped outside of the U.S. do not qualify for return, refund, or exchange. Warranty is excluded.
- Pre-Paid Calibration coupons do not qualify for refund or exchange.
What If I Ordered A Product By Error?
If I Received A Damaged Or A Broken Item?
- If an item arrived damaged or broken, please fill out a Return Form and send clear photos with good lighting of the defect for each affected item to info@pipette.com. Please include pictures of the packaging and the damaged product. We will provide you with a free shipping label to return such items and process a replacement order.
- If the item requiring replacement is not in stock, you may be sent a substitute of equal value.
What do I do if My Product Is defective within the designated warranty period?
- If an item is defective within the designated warranty period, you must contact us immediately to receive instructions on how to proceed. Warranty-related issues are typically handled on a case-by-case basis due to various manufacturer warranty guidelines.
- We typically repair warranty items at no additional charge. If the item is not repairable, we will provide an exchange at no additional charge.
- In the event the customer is responsible for the product defect, the product will not be eligible for warranty repair, and we will contact you with a repair estimate.
- There may be additional charges for broken parts deemed unrepairable due to excessive force/incorrect usage.
- Autoclave damage due to higher than tolerated temperatures, pressurized steam, or extended exposure time (per pipette specification) will result in a voided warranty. We are unable to repair this damage.
- Autoclave damage is typically non repairable and requires purchase of a replacement product, at customers expense.
What Do I Do If I have Received An Item That I Did Not Order Or A Duplicate?
- Please contact us immediately via our “Return / Exchange Request” form and we will provide you with a free shipping label to return the incorrect item/s. We will then send you the correct item(s) immediately.
- If you do not return the product, you will be billed for it.
We’re here to help. Below are the top 5 most common questions we hear from labs like yours—and how we can help.
Have More Questions?
Do not hesitate to contact our team and ask your question:

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Note: this offer is available April 1-30, the coupon is applicable only once.
Stay cosmic (and precise)!